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gp2 293 packaging cells  (TaKaRa)


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    Structured Review

    TaKaRa gp2 293 packaging cells
    Gp2 293 Packaging Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 372 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gp2+293/Retro-X+Universal+Packaging+System/pm41742411-396-6-9
    Average 96 stars, based on 372 article reviews
    gp2 293 packaging cells - by Bioz Stars, 2026-10
    96/100 stars

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    Article Title: Precision off-the-shelf natural killer cell therapies for oncology with logic-gated gene circuits.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Affymetrix Human Genome U133 Plus 2.0 Array datasets (various) Gene Expression Omnibus (https://www.ncbi.nlm.nih.gov/geo/) GSE13159, GSE15434, GSE17054, GSE24006, GSE28490, GSE28491, GSE42519, GSE49910, GSE63270, GSE6891, GSE93777 Human (NCBITaxon:9606) ‘‘cell surface’’ proteins Gene Ontology Resource (https://geneontology.org) GO:0009986 Human (NCBITaxon:9606) ‘‘membrane’’ proteins Gene Ontology Resource (https://geneontology.org) GO:0016020 Antibody-based annotations of protein localization Human Protein Atlas (https:// www.proteinatlas.org/) N/A AML bulk RNAseq data https://www.cancer.gov/ccg/research/ genome-sequencing/tcga N/A The gene and gene product information of KLRG1 Uniprot (https://www.uniprot.org/) Q96E93 The gene and gene product information of BTLA Uniprot (https://www.uniprot.org/) Q7Z6A9 The gene and gene product information of KIR3DL1 Uniprot (https://www.uniprot.org/) P43629 The gene and gene product information of NKG2A Uniprot (https://www.uniprot.org/) P26715 The gene and gene product information of SIGLEC-2 Uniprot (https://www.uniprot.org/) P20273 The gene and gene product information of SIGLEC-10 Uniprot (https://www.uniprot.org/) Q96LC7 The gene and gene product information of LIR-2 Uniprot (https://www.uniprot.org/) Q8N423 The gene and gene product information of LIR-3 Uniprot (https://www.uniprot.org/) O75022 The gene and gene product information of LAIR1 Uniprot (https://www.uniprot.org/) Q6GTX8 The gene and gene product information of KIR2DL1 Uniprot (https://www.uniprot.org/) P43626 The gene and gene product information of LIR1 Uniprot (https://www.uniprot.org/) Q8NHL6 The gene and gene product information of CD33 Uniprot (https://www.uniprot.org/) P20138 The gene and gene product information of FLT3 Uniprot (https://www.uniprot.org/) P36888 The gene and gene product information of EMCN Uniprot (https://www.uniprot.org/) Q9ULC0 Experimental models: Cell lines Human: SEM (ALL, female) DSMZ ACC 546 Human: MOLM13 (AML, male) AddexBio C0003003 Human: MV4-11 (AML/ALL, male) ATCC CRL-9591 Human: Lenti-X 293T (transformed, female) Takara 632180 Human: GP2-293 (transformed, female) Takara 631458 Experimental models: Organisms/strains NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(IL15)1Sz/SzJ Jackson Laboratories 030890 Recombinant DNA Gene circuit constructs This paper N/A Antigen expression constructs This paper N/A Software and algorithms Prism GraphPad https://www.graphpad.com/ scientific-software/prism/

    Article Title: Cell-type specific, inducible and acute degradation of targeted protein in mice by two degron systems
    Article Snippet: HEK293T (Riken BRC, Cat no. RCB2202), GP2-293 (Takara Bio, Cat no. 631530), Jurkat (Riken BRC, Cat no. RCB0806), and MC38 (provided by Dr. James P. Allison) cell lines were used in the study.

    Stable Transfection:

    Article Title: Cooperation between Intrinsically Disordered Regions regulates CBP condensate behaviour
    Article Snippet: All DNA constructs used are available from Addgene. .. Human Embryonic Kidney cells stably expressing the SV40 large T antigen (HEK293T, ATCC) and GP2-293 (Clontech) cells were cultured in complete DMEM (10% FBS, 1% Penicillin-Streptomycin (Gibco)) and grown in 5% CO 2 at 37°C. .. HEK293T cells containing stable doxycycline inducible CBP-GFP, were cultured in complete DMEM (10% Tetracycline free FBS, 1% Penicillin-Streptomycin) including 2 mg/ml G418 (Generon) and 50ug/ml hygromycin (ThermoFisher).

    Expressing:

    Article Title: Cooperation between Intrinsically Disordered Regions regulates CBP condensate behaviour
    Article Snippet: All DNA constructs used are available from Addgene. .. Human Embryonic Kidney cells stably expressing the SV40 large T antigen (HEK293T, ATCC) and GP2-293 (Clontech) cells were cultured in complete DMEM (10% FBS, 1% Penicillin-Streptomycin (Gibco)) and grown in 5% CO 2 at 37°C. .. HEK293T cells containing stable doxycycline inducible CBP-GFP, were cultured in complete DMEM (10% Tetracycline free FBS, 1% Penicillin-Streptomycin) including 2 mg/ml G418 (Generon) and 50ug/ml hygromycin (ThermoFisher).

    Article Title: Exploring T Cell Reactivity to Gliadin in Young Children with Newly Diagnosed Celiac Disease
    Article Snippet: The transduced 5KC hybridomas were sorted by GFP expression and TCR expression was confirmed by staining with anti-mouse TCR β antibody (clone H57-597, BD Biosciences). .. Alternatively, the expression of plasmids in the pMSCVpuro retroviral vector (Clontech) was transfected to AmphoPack-293 or GP2-293 (VSV-G envelope) packaging cells (Clontech) to produce retrovirus and mouse 5KC cells were spin-infected with retroviral supernatants and cultured with puromycin. .. TCR expression was confirmed in isolated single clones by staining with anti-mouse CD3 (clone 145-2C11; BD Biosciences) or mouse TCR β antibody (clone H57-597; BD Biosciences).

    Cell Culture:

    Article Title: Cooperation between Intrinsically Disordered Regions regulates CBP condensate behaviour
    Article Snippet: All DNA constructs used are available from Addgene. .. Human Embryonic Kidney cells stably expressing the SV40 large T antigen (HEK293T, ATCC) and GP2-293 (Clontech) cells were cultured in complete DMEM (10% FBS, 1% Penicillin-Streptomycin (Gibco)) and grown in 5% CO 2 at 37°C. .. HEK293T cells containing stable doxycycline inducible CBP-GFP, were cultured in complete DMEM (10% Tetracycline free FBS, 1% Penicillin-Streptomycin) including 2 mg/ml G418 (Generon) and 50ug/ml hygromycin (ThermoFisher).

    Article Title: A combinatorial therapeutic approach to enhance FLT3-ITD AML treatment.
    Article Snippet: .. Cell lines and cell culture The cell lines used in the study included MV4-11 (ATCC, CRL-9591), HEK-293T (ATCC, CRL-3216), MOLM-13 (DSMZ, ACC 554), OCI-AML2 (DSMZ, ACC 99), OCI-AML3 (DSMZ, ACC 582), and GP2-293 (Takara). .. MV4-11, MOLM-13, OCI-AML2, and OCIAML3 cells were cultured in RPMI 1640 (Gibco).

    Article Title: Exploring T Cell Reactivity to Gliadin in Young Children with Newly Diagnosed Celiac Disease
    Article Snippet: The transduced 5KC hybridomas were sorted by GFP expression and TCR expression was confirmed by staining with anti-mouse TCR β antibody (clone H57-597, BD Biosciences). .. Alternatively, the expression of plasmids in the pMSCVpuro retroviral vector (Clontech) was transfected to AmphoPack-293 or GP2-293 (VSV-G envelope) packaging cells (Clontech) to produce retrovirus and mouse 5KC cells were spin-infected with retroviral supernatants and cultured with puromycin. .. TCR expression was confirmed in isolated single clones by staining with anti-mouse CD3 (clone 145-2C11; BD Biosciences) or mouse TCR β antibody (clone H57-597; BD Biosciences).

    Retroviral:

    Article Title: Exploring T Cell Reactivity to Gliadin in Young Children with Newly Diagnosed Celiac Disease
    Article Snippet: The transduced 5KC hybridomas were sorted by GFP expression and TCR expression was confirmed by staining with anti-mouse TCR β antibody (clone H57-597, BD Biosciences). .. Alternatively, the expression of plasmids in the pMSCVpuro retroviral vector (Clontech) was transfected to AmphoPack-293 or GP2-293 (VSV-G envelope) packaging cells (Clontech) to produce retrovirus and mouse 5KC cells were spin-infected with retroviral supernatants and cultured with puromycin. .. TCR expression was confirmed in isolated single clones by staining with anti-mouse CD3 (clone 145-2C11; BD Biosciences) or mouse TCR β antibody (clone H57-597; BD Biosciences).

    Plasmid Preparation:

    Article Title: Exploring T Cell Reactivity to Gliadin in Young Children with Newly Diagnosed Celiac Disease
    Article Snippet: The transduced 5KC hybridomas were sorted by GFP expression and TCR expression was confirmed by staining with anti-mouse TCR β antibody (clone H57-597, BD Biosciences). .. Alternatively, the expression of plasmids in the pMSCVpuro retroviral vector (Clontech) was transfected to AmphoPack-293 or GP2-293 (VSV-G envelope) packaging cells (Clontech) to produce retrovirus and mouse 5KC cells were spin-infected with retroviral supernatants and cultured with puromycin. .. TCR expression was confirmed in isolated single clones by staining with anti-mouse CD3 (clone 145-2C11; BD Biosciences) or mouse TCR β antibody (clone H57-597; BD Biosciences).

    Transfection:

    Article Title: Exploring T Cell Reactivity to Gliadin in Young Children with Newly Diagnosed Celiac Disease
    Article Snippet: The transduced 5KC hybridomas were sorted by GFP expression and TCR expression was confirmed by staining with anti-mouse TCR β antibody (clone H57-597, BD Biosciences). .. Alternatively, the expression of plasmids in the pMSCVpuro retroviral vector (Clontech) was transfected to AmphoPack-293 or GP2-293 (VSV-G envelope) packaging cells (Clontech) to produce retrovirus and mouse 5KC cells were spin-infected with retroviral supernatants and cultured with puromycin. .. TCR expression was confirmed in isolated single clones by staining with anti-mouse CD3 (clone 145-2C11; BD Biosciences) or mouse TCR β antibody (clone H57-597; BD Biosciences).

    Modification:

    Article Title: Langat virus inhibits the gp130/JAK/STAT signaling by reducing the gp130 protein level.
    Article Snippet: .. Vero (ATCC CRL‐81), SVG cells (ATCC CRL‐8621), HEK293 (ATCC CRL‐1573), HEK293T (ATCC CRL‐3216TM), and GP2‐293 (Takara Bio USA, Inc., Mountain View, CA) cells were maintained in Dulbecco's modified Eagle's medium (DMEM) that is supplemented with 10% fetal bovine serum (FBS). ..



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